Colisure

Yes, the Colisure Test is U.S. Environmental Protection Agency (EPA) approved as a method for total coliforms and E. coli detection in drinking water and for E. coli detection in groundwater. 

The Colisure Test detects coliforms and E. coli at 1 organism/100 mL. 

 The shelf life for the Colisure Test is up to 12 months from date of manufacture. 

  Store at 2–25°C and away from light. 

There is no comparator for the Colisure Test. For comparison, use a negative control when interpreting results. 

The reagent system was designed to suppress non-coliform CPRG or MUG positive bacteria for at least 48 hours. If incubation extends past 48 hours, it is possible that such bacteria could overcome the reagent and react with CPRG or MUG. When incubation exceeds 48 hours, only negative results are valid. Positive results must be confirmed. 

Check with your local, state, and/or federal authorities for proper disposal of bacteriological biohazard materials at your facility. 

If an inoculated Colisure Test sample is inadvertently incubated over 48 hours, lack of red to magenta color is a valid negative test; a red to magenta color after 48 hours is not valid and should be repeated. 

An inoculated Colisure Test sample that is positive for coliforms and E. coli before 24 hours is a valid positive test for both coliforms and E. coli. If the sample is positive before 24 hours for coliforms only, the sample is a valid positive test for total coliforms. However, the sample should be incubated for a longer period (at least 24 hours, but not greater than 48 hours total time) to determine whether E. coli are present. 

Yes, when using the Colisure Test with Quanti-Tray, Quanti-Tray/2000 or any multiple-tube format, be sure to use IDEXX Antifoam Solution or a vessel with antifoam. 

The Colisure reagent powder should be tan in color; it should be dry and free-flowing. Any concerns about the color or integrity of the powder should be directed to IDEXX Technical Support.