Pseudalert
24-hour detection of Pseudomonas aeruginosa
- Detects P. aeruginosa in 24 hours with no additional confirmation steps.
- The ISO international standard for 24-hour detection of P. aeruginosa in water - ISO: 16266-2:2018.
- Method 9213G in Standard Methods for Examination of Water and Wastewater.
- Minimizes the risk of false positives from nontarget organisms.
- Same easy-to-use platform as the Colilert Test.
- Safeguard against "hot-tub rash," "swimmer’s ear," and a host of other infections, avoiding illness in sensitive populations.
The Science
How the Pseudalert Test works
The Pseudalert Test detects the presence of Pseudomonas aeruginosa in water samples.
The test is based on a bacterial enzyme detection technology that signals the presence of P. aeruginosa through the hydrolysis of a substrate in the Pseudalert reagent. P. aeruginosa cells rapidly grow and reproduce using the rich supply of amino acids, vitamins, and other nutrients present in the Pseudalert reagent. Actively growing strains of P. aeruginosa have an enzyme that cleaves the substrate in the reagent to produce blue fluorescence under ultraviolet light.
Pseudalert detects Pseudomonas aeruginosa at 1 cfu per 100 mL water sample within 24 hours.
Presence/Absence (P/A)
Step 1
Step 1
Step 1
Add reagent to sample (sample will turn yellow), and then incubate at 38°C±0.5°C for 24–28 hours.
Step 2
Step 1
Step 1
Read under ultraviolet (UV) light. Blue fluorescence indicates presence of P. aeruginosa. See the Pseudalert Quick Reference Guide (PDF) for tips on reading fluorescence.
Quantification
Step 1
Step 1
Step 1
Add reagent to sample (sample will turn yellow).
Step 2
Step 1
Step 1
Add IDEXX Antifoam Solution to vessel.
Step 3
Step 1
Step 3
Pour sample into Quanti-Tray (counts from 1–200) or Quanti-Tray/2000 (counts from 1–2,419).
Step 4
Step 4
Step 3
Seal using a Quanti-Tray Sealer, and then incubate at 38°C ± 0.5°C for 24–28 hours.
Step 5
Step 4
Step 5
Read under ultraviolet (UV) light. Blue fluorescence indicates the presence ofP. aeruginosa. Count the positive (blue fluorescent) wells and refer to MPN table. See the Pseudalert Quick Reference Guide (PDF) for tips on reading fluorescence.